Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology.

gentur

Infusion of various hematopoietic stem cell populations and ex vivo expanded endothelial progenitor cells augments neovascularization of tissue after ischemia and contributes to reendothelialization after endothelial harm, thereby, offering a novel therapeutic possibility. However, controversy exists with respect to the identification and the origin of endothelial progenitor cells. Overall, there’s consensus that endothelial progenitor cells can derive from the bone marrow and that CD133/VEGFR2 cells symbolize a inhabitants with endothelial progenitor capability.

LEC Chemokine(LEC67) Antibody

BNCA0067-250 250uL
EUR 459.6
Description: Primary antibody against LEC Chemokine(LEC67), APC conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC800066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF680 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC800066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF680 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC800067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF680 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC800067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), CF680 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC810066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF680R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC810066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF680R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC810067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF680R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC810067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), CF680R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC940066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF594 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC940066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF594 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC940067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF594 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC940067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), CF594 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC880066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF488A conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC880066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF488A conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC880067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF488A conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC880067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), CF488A conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC430066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF543 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC430066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF543 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC430067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF543 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC430067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), CF543 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC400066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF640R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC400066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF640R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC400067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF640R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC400067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), CF640R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC050066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF405M conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC050066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF405M conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC050067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF405M conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC050067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), CF405M conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCAP0066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCAP0066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCAP0067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCAP0067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCB0066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), Biotin conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCB0066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), Biotin conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCB0067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), Biotin conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCB0067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), Biotin conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCR0066-250 250uL
EUR 459.6
Description: Primary antibody against LEC Chemokine(LEC66), RPE conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCR0067-250 250uL
EUR 459.6
Description: Primary antibody against LEC Chemokine(LEC67), RPE conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNUB0066-100 100uL
EUR 250.8
Description: Primary antibody against LEC Chemokine(LEC66), Concentration: 0.2mg/mL

LEC Chemokine(LEC66) Antibody

BNUB0066-500 500uL
EUR 549.6
Description: Primary antibody against LEC Chemokine(LEC66), Concentration: 0.2mg/mL

LEC Chemokine(LEC67) Antibody

BNUB0067-100 100uL
EUR 250.8
Description: Primary antibody against LEC Chemokine(LEC67), Concentration: 0.2mg/mL

LEC Chemokine(LEC67) Antibody

BNUB0067-500 500uL
EUR 549.6
Description: Primary antibody against LEC Chemokine(LEC67), Concentration: 0.2mg/mL

LEC Chemokine(LEC66) Antibody

BNCP0066-250 250uL
EUR 459.6
Description: Primary antibody against LEC Chemokine(LEC66), PerCP conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCP0067-250 250uL
EUR 459.6
Description: Primary antibody against LEC Chemokine(LEC67), PerCP conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNUM0066-50 50uL
EUR 474
Description: Primary antibody against LEC Chemokine(LEC66), 1mg/mL

LEC Chemokine(LEC67) Antibody

BNUM0067-50 50uL
EUR 474
Description: Primary antibody against LEC Chemokine(LEC67), 1mg/mL

LEC Chemokine(LEC66) Antibody

BNCH0066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCH0066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCH0067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCH0067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC040066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF405S conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC040066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF405S conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC040067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF405S conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC040067-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC67), CF405S conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC550066-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC66), CF555 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC550066-500 500uL
EUR 652.8
Description: Primary antibody against LEC Chemokine(LEC66), CF555 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC550067-100 100uL
EUR 238.8
Description: Primary antibody against LEC Chemokine(LEC67), CF555 conjugate, Concentration: 0.1mg/mL

However, rising proof means that there are further bone marrow-derived cell populations (eg, myeloid cells, “facet inhabitants” cells, and mesenchymal cells) and non-bone marrow-derived cells, which additionally can provide rise to endothelial cells.

gentur

The characterization of the totally different progenitor cell populations and their practical properties are mentioned. Mobilization and endothelial progenitor cell-mediated neovascularization is critically regulated. Stimulatory (eg, statins and train) or inhibitory components (danger components for coronary artery illness) modulate progenitor cell ranges and, thereby, have an effect on the vascular restore capability.

Moreover, recruitment and incorporation of endothelial progenitor cells requires a coordinated sequence of multistep adhesive and signaling occasions together with adhesion and migration (eg, by integrins), chemoattraction (eg, by SDF-1/CXCR4), and lastly the differentiation to endothelial cells. This evaluate summarizes the mechanisms regulating endothelial progenitor cell-mediated neovascularization and reendothelialization.

 

Bone marrow stromal stem cells: nature, biology, and potential functions.

  1. Bone marrow stromal cells are progenitors of skeletal tissue parts reminiscent of bone, cartilage, the hematopoiesis-supporting stroma, and adipocytes. In addition, they might be experimentally induced to endure unorthodox differentiation, probably forming neural and myogenic cells. As such, they symbolize an necessary paradigm of post-natal nonhematopoietic.
  2. stem cells, and a straightforward supply for potential therapeutic use. Along with an outline of the fundamentals of their biology, we talk about right here their potential nature as parts of the vascular wall, and the prospects for their use in native and systemic transplantation and gene remedy.
  • Fluorescent probes are one of many cornerstones of real-time imaging of dwell cells and a robust instrument for cell biologists. They present excessive sensitivity and nice versatility whereas minimally perturbing the cell underneath investigation.

 

  • Genetically-encoded reporter constructs which can be derived from fluorescent proteins are main a revolution within the real-time visualization and monitoring of varied mobile occasions. Recent advances embody the continued improvement of ‘passive’ markers for the measurement of biomolecule expression and localization in dwell cells, and ‘lively’ indicators for monitoring extra complicated mobile processes reminiscent of small-molecule-messenger dynamics, enzyme activation and protein-protein interactions.

 

  • The mobile processes that govern neuronal operate are extremely complicated, with many fundamental cell organic pathways uniquely tailored to carry out the flowery info processing achieved by the mind. This is especially evident within the trafficking and regulation of membrane proteins to and from synapses, which is usually a lengthy distance away from the cell physique and quantity within the hundreds.

JAK2 JH2 Tracer

HY-102055 50mg
EUR 5515.2

CytoFixâ„¢ BCECF, AM *Optimized for long term cellular pH tracking*

21200-1mg 1 mg
EUR 318
Description: Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes.

CytoFix™ BCECF, AM *Optimized for long term cellular pH tracking*

21200 1 mg
EUR 318

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 985.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 4027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 1027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 4179.6
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 866.4
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 3463.2
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145 384 assays
EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145-5 5 x 384 assays
EUR 4681.2
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

293/GFP Cell Line

AKR-200 1 vial
EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation), Trial Size

CBA-130-T 24 assays
EUR 463.2
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 582
Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix.

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 490.8
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Radius 24-Well Cell Migration Assay

CBA-125 24 assays
EUR 602.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 24-Well Cell Migration Assay

CBA-125-5 5 x 24 assays
EUR 2362.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 686.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2697.6
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037 48 assays
EUR 622.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037-5 5 x 48 assays
EUR 2454
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 637.2
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

CytoSelect 24-well Cell Invasion, Fluorometric

CBA-111 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 96-well Cell Invasion, Fluorometric

CBA-112 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells.

CytoSelect Cell Viability and Cytotoxicity Assay

CBA-240 96 assays
EUR 470.4
Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

CytoSelect 24-Well Cell Co-Culture System

CBA-160 24 assays
EUR 525.6
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions.

CytoSelect 24-Well Cell Co-Culture System

CBA-160-5 5 x 24 assays
EUR 2078.4
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions.

CytoSelect 48-Well Cell Contraction Assay Kit

CBA-5021 48 assays
EUR 762
Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction.

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation.  The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

CytoSelect 24-well Cell Invasion Assay, Colorimetric

CBA-110 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

CytoSelect 24-well Laminin Cell Invasion, Colorimetric

CBA-110-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

CytoSelect 24-well Collagen Cell Invasion, Colorimetric

CBA-110-COL 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.
  • The regulation of neurotransmitter receptors, such because the AMPA-type glutamate receptors (AMPARs), the most important excitatory neurotransmitter receptors within the mind, is an important mechanism for the modulation of synaptic transmission. The ranges of AMPARs at synapses are very dynamic, and it’s these plastic modifications in synaptic operate which can be thought to underlie info storage within the mind.

 

  • Thus, understanding the mobile equipment that controls AMPAR trafficking can be crucial for understanding the mobile foundation of habits in addition to many neurological ailments. Here we describe the life cycle of AMPARs, from their biogenesis, by way of their journey to the synapse, and in the end by way of their demise, and talk about how the modulation of this course of is crucial for mind operate.

 

  • Adult skeletal muscle has a outstanding capability to regenerate following myotrauma. Because grownup myofibers are terminally differentiated, the regeneration of skeletal muscle is basically depending on a small inhabitants of resident cells termed satellite tv for pc cells. Although this inhabitants of cells was recognized 40 years in the past, little is thought concerning the molecular phenotype or regulation of the satellite tv for pc cell.

 

  • The use of cell tradition strategies and transgenic animal fashions has improved our understanding of this distinctive cell inhabitants; nonetheless, the capability and potential of those cells stay ill-defined. This evaluate will spotlight the origin and distinctive markers of the satellite tv for pc cell inhabitants, the regulation by development components, and the response to physiological and pathological stimuli. We conclude by highlighting the potential therapeutic makes use of of satellite tv for pc cells and figuring out future analysis objectives for the research of satellite tv for pc cell biology.

Leave A Comment